‘Quadruple helix’ DNA in humans

January 20, 2013
By

A representation of the four-stranded structure (L) and fluorescent markers reveal its presence inside cells (R) A representation of the four-stranded structure (L) with fluorescent markers revealing its presence inside cells (R)

Cambridge University scientists say they have seen four-stranded DNA at work in human cells for the first time.

The famous “molecule of life”, which carries our genetic code, is more familiar to us as a double helix.

But researchers tell the journal Nature Chemistry that the “quadruple helix” is also present in our cells, and in ways that might possibly relate to cancer.

They suggest that control of the structures could provide novel ways to fight the disease.

“The existence of these structures may be loaded when the cell has a certain genotype or a certain dysfunctional state,” said Prof Shankar Balasubramanian from Cambridge’s department of chemistry.

“We need to prove that; but if that is the case, targeting them with synthetic molecules could be an interesting way of selectively targeting those cells that have this dysfunction,” he told BBC News.

Tag and track

It will be exactly 60 years ago in February that James Watson and Francis Crick famously burst into the pub next to their Cambridge laboratory to announce the discovery of the “secret of life”.

What they had actually done was describe the way in which two long chemical chains wound up around each other to encode the information cells need to build and maintain our bodies.

Today, the pair’s modern counterparts in the university city continue to work on DNA’s complexities.

Balasubramanian’s group has been pursuing a four-stranded version of the molecule that scientists have produced in the test tube now for a number of years.

It is called the G-quadruplex. The “G” refers to guanine, one of the four chemical groups, or “bases”, that hold DNA together and which encode our genetic information (the others being adenine, cytosine, and thymine).

The G-quadruplex seems to form in DNA where guanine exists in substantial quantities.

And although ciliates, relatively simple microscopic organisms, have displayed evidence for the incidence of such DNA, the new research is said to be the first to firmly pinpoint the quadruple helix in human cells.

‘Funny target’

The team, led by Giulia Biffi, a researcher in Balasubramaninan’s lab, produced antibody proteins that were designed specifically to track down and bind to regions of human DNA that were rich in the quadruplex structure. The antibodies were tagged with a fluorescence marker so that the time and place of the structures’ emergence in the cell cycle could be noted and imaged.

This revealed the four-stranded DNA arose most frequently during the so-called “s-phase” when a cell copies its DNA just prior to dividing.

Prof Balasubramaninan said that was of key interest in the study of cancers, which were usually driven by genes, or oncogenes, that had mutated to increase DNA replication.

If the G-quadruplex could be implicated in the development of some cancers, it might be possible, he said, to make synthetic molecules that contained the structure and blocked the runaway cell proliferation at the root of tumours.

“We’ve come a long way in 10 years, from simple ideas to really seeing some substance in the existence and tractability of targeting these funny structures,” he told the BBC.

“I’m hoping now that the pharmaceutical companies will bring this on to their radar and we can perhaps take a more serious look at whether quadruplexes are indeed therapeutically viable targets.”

Prof Shankar BalasubramanianProf Shankar Balasubramanian in front of a painting by artist Annie Newman that represents quadruplex DNA

[email protected] and follow me on Twitter: @BBCAmos

Article source: http://www.bbc.co.uk/news/science-environment-21091066#sa-ns_mchannel=rss&ns_source=PublicRSS20-sa The feed :

Tags:

Sponsored By:

The Deeter Group

Deeter Electronics Ltd. | Deeter Electronics Inc | Deeter Group Asia | Deeter Group Germany | Deeter Group Corporate

Head & Registered Office:
Deeter Electronics Ltd.
Deeter House,
Valley Road,
Hughenden Valley,
Bucks. HP14 4LW

Tel: +44 (0) 1494 566 046
Fax: +44 (0) 1494 563 961
E-mail: [email protected]


The Deeter Group with products ranging from: , , , continuous , 4-20mA , , , , , , , a , wireless sensor and much more.

If you want a standard or a custom level switch, float switch, level sensor, or wireless sensor, contact us via email [email protected] or call us now on 01494 566 046.

Company Name: The Deeter Group

Location: Hughenden Valley, UK

Deeter Electronics USA
Deeter Electronics USA
Wireless sensor system | Radio telemetry | Level switches | Level sensors | Liquid level sensor | Float switches | Controllers & indicators | Industrial weighing equipment | Electronic circuit design & manufacture | Software design | Reed relays | Ultrasonic level flow sludge & proximity sensors | Proximity sensors & switches | Reed switches | Litz wire | Wago Terminals | Mechanical & magnetic floats | Boiler control |

Deeter Electronics UK
Wireless sensor system | Radio telemetry | Level switches | Level sensors | Liquid level sensor | Float switches | Controllers & indicators | Industrial weighing equipment | Electronic circuit design & manufacture | Software design | Reed relays | Ultrasonic level flow sludge & proximity sensors | Proximity sensors & switches | Reed switches | Litz wire | Wago Terminals | Mechanical & magnetic floats | Boiler control |

Deeter Electronics Europe
Wireless sensor system | Radio telemetry | Level switches | Level sensors | Liquid level sensor | Float switches | Controllers & indicators | Industrial weighing equipment | Electronic circuit design & manufacture | Software design | Ultrasonic level flow sludge & proximity sensors | Proximity sensors & switches | Reed switches | Litz wire | Wago Terminals | Mechanical & magnetic floats | Boiler control |

Sensor Magazine Websites
Explosion Proof Sensors UK | Explosion Proof Sensors | Water Level Measurement | Liquid Level Sensing | Liquid Level Measurement | Liquid Level Sensors UK | Liquid Level Sensors Europe | Wireless Sensors | Sensor Magazine | Industrial Sensors



Water use